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The Illumina process and reagents were used to perform the library prep, with Twelve menstrual cycles involving PCR sound. Your local library ended up sequenced utilizing an Illumina GAIIx and also assessed since defined in record 3. ChIP-qPCR assays Chips assays ended up executed as described inside the ChIP-seq segment, with the exception that 25 ��g equivalents regarding Genetic was used for ev
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The Illumina method and reagents were chosen to perform the particular selection preparing, together with 14 cycles regarding PCR sound. Your local library were sequenced having an Illumina GAIIx and examined since explained in document Three. ChIP-qPCR assays Chips assays have been done since defined from the ChIP-seq area, other than 30 ��g equivalents of Genetic make-up was utilized per ChIP re
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With SLE and has been involved in the development of murine lupus [57, 58]. A number of studies have linked genetic variants in OPN with SLE susceptibility, clinical manifestations of SLE; and high IFN levels in SLE affected males and young-onset female lupus patients [13, 59]. OPN interacts with the MyD88 adaptor protein downstream of TLR ligation, and is an important molecule for IFN- production
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