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Wo groups.Before SCI there were no significant differences among 5 groups in paw withdrawal threshold in the tactile allodynia test with von Frey filaments, or in the percentage of withdrawal responses in the acetone test for cold allodynia (Figure 4, P > 0.05). Figure 4A shows changes in von Frey responses over the 7 week time course following viral vector injections (overall F (df 4,9) = 7.941,
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Alent of the human acupoint Huantiao (GB30) was chosen for bilateral needling based on traditional Chinese medicine (TCM) meridian theory [14], on its successful use in our previous studies, and on studies by others [3,15,16]. Based on our previous point-specificity study [3], EA produced better anti-hyperalgesia at GB30 than at acupoint Waiguan (the fifth acupoint on the Triple Energizer Meridian
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S was attached to the handles of the needles. The needles and electrodes were stabilized with adhesive tape. EA stimulation was delivered by an electrical stimulator (A300 Pulsemaster, World Precision Instruments) via an isolator (A360D Stimulus Isolator, World Precision Instruments) that delivers steady direct current. This bilateral, cross-limb connection has been used previously by our team and